The DetectX® Estrone ELISA Kit is designed to quantitatively measure estrone present in extracted dried fecal samples, urine and tissue culture media samples. The kit is unique as it measures both non-conjugated estrone, estrone-3-sulfate and estrone 3-glucuronide in urine and fecal samples with almost equal affinity, allowing for non-invasive testing of this steroid. An estrone standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate. An estrone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to estrone to each well. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound estrone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is read at 450nm.
Arbor Assays公司成立于2007年，位于密歇根州的安娜堡，公司的三位主创始人均为有多年科研经验的生命和临床科学家。其宗旨是为临床使用的重要生物分子建立高质量的检测和免疫测试产品。在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。
Arbor Assays公司的主要产品包括：
DetectX® Kits
Inhibitors & Activators
AbX® Antibodies & Affinity Resins
Detection Systems
Components & Ancillary Reagents
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