The DetectX® Estrone-3-Glucuronide (E1G) ELISA Kit uses a specifically generated antibody to measure E1G and its metabolites in urine and fecal samples, or in extracted serum and plasma. The kit will quantitatively measure E1G present in diluted buffer samples and tissue culture media samples. An E1G standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An E1G-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to E1G to each well. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound E1G-peroxidase conjugate. After a short incubation, the reaction is read at 450nm wavelength.
Arbor Assays公司成立于2007年，位于密歇根州的安娜堡，公司的三位主创始人均为有多年科研经验的生命和临床科学家。其宗旨是为临床使用的重要生物分子建立高质量的检测和免疫测试产品。在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。
Arbor Assays公司的主要产品包括：
DetectX® Kits
Inhibitors & Activators
AbX® Antibodies & Affinity Resins
Detection Systems
Components & Ancillary Reagents
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