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主营:酶活性试剂盒,免疫检测试剂盒
℡ 4000-520-616
℡ 4000-520-616
ARBOR ASSAYS/Glutathione (GSH) Fluorescent 384-Well Detection Kit/K006-F1D/F1D
产品编号:K006-F1D
市  场 价:¥9700.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$485.00
品      牌: ARBOR ASSAYS
公      司:ARBOR ASSAYS
公司分类:
ARBOR ASSAYS/Glutathione (GSH) Fluorescent 384-Well Detection Kit/K006-F1D/F1D
商品介绍
  • MostSensitive
  • MultiSpecies
  • USE-MeasureFreeorOxidizedGlutathionein15Minutes.
  • SAMPLE-Lysates,WB,RBCs,Serum,Plasma,UrineandTissue
  • SAMPLES/KIT-~183(FreeandOxidized)inDuplicate
  • STABILITY-Stable4˚CLiquidReagents

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TheDetectX®Glutathione(GSH)FluorescentDetectionkitutilizesaproprietarynon-fluorescentmolecule,ThioStar®,thatcovalentlybindstothefreethiolgrouponGSHtoyieldahighlyfluorescentproduct.AftermixingthesampleorstandardwithThioStar®andincubatingatroomtemperaturefor15minutes.Thiskituses2384-wellplatesthatallowthemeasurementofabout375samplesinduplicateforeitherGSHorGSSGcontent,ormeasureabout183samplesinduplicateforbothGSHandGSSG.Thesignalproducedisreadat510nminafluorescentplatereaderwithexcitationat390nm.SampleGSSGisdeterminedbyadditionofareactionmixtureconvertsalltheGSSGintofreeGSH,whichthenreactswithThioStar®.

Glutathione(GSH)isthehighestconcentrationnon-proteinthiolinmammaliancellsandispresentinconcentrationsof0.5to10mM.GSHplaysakeyroleinmanyBIOLOGicalprocesses,includingthesynthesisofproteinsandDNA,thetransportofaminoacids,andtheprotectionofcellsagainstoxidation.Harmfulhydrogenperoxidecellularlevelsareminimizedbytheenzymeglutathioneperoxidase(GP)usingGSHasareductant.TheoxidizedGSHdimer,GSSG,isformedfromGSHandperoxidebytheGPreaction.AnimportantroleofGSSGintheNFkBactivatingsignalcascadeissuggestedbythefactthatthepotentNFkBinducerTPAincreasesintracellularGSSGlevelsandGSSG/GSHratios.

384-Well Glutathione Fluorescent Standard Curve

 

DataReductionTemplatebyMyAssays

品牌介绍

Arbor Assays公司成立于2007年,位于密歇根州的安娜堡,公司的三位主创始人均为有多年科研经验的生命和临床科学家。其宗旨是为临床使用的重要生物分子建立高质量的检测和免疫测试产品。在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。

 

Arbor Assays公司的主要产品包括:

DetectX® Kits

Inhibitors & Activators

AbX® Antibodies & Affinity Resins

Detection Systems

Components & Ancillary Reagents


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